Coding

Part:BBa_M45172:Design

Designed by: Andrew Alarcon Parker   Group: UtahState_BE5930_-_S14   (2016-04-12)


cadA gene for Lysine decarboxylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1104
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 490
    Illegal BamHI site found at 809
    Illegal BamHI site found at 1728
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1081
    Illegal BsaI.rc site found at 1159
    Illegal BsaI.rc site found at 2068
    Illegal SapI.rc site found at 46


Design Notes

Sequence has strangely enough been optimized for expression within the Felix cattus genome. It is suggested that it be optimized again for the desired clone species. Prefix and suffix RE sites have been removed from the sequence. Again this part has not been tested and is HYPOTHETICAL only.


Source

From E.coli (K12)cds. Sequence originally published by Meng,S.Y. and Bennett,G.N. in "Nucleotide sequence of the Escherichia coli cad operon: a system for neutralization of low extracellular pH" (1992)

References